FIGURE 4.

GsSRK-f and GsSRK-t overexpression in alfalfa contributes to increased salt tolerance. (A) Shoot growth performance of the WT and transgenic alfalfa under salt stress. (B) Root growth performance of the WT and transgenic alfalfa under salt stress. The WT and transgenic alfalfa seedlings showing similar growth performance were irrigated with 1/8 Hoagland solution containing either 0 or 200 or 300 mM NaCl every 2 days for a total of 18 days. (C) Numbers of branches of the WT and transgenic alfalfa under control conditions and salt stress. (D) Shoot length of the WT and transgenic alfalfa under control conditions and salt stress. (E) Fresh weight of the WT and transgenic alfalfa under control conditions and salt stress. Ten seedlings of each line were used for each experiment. Data are means ± SE (n ≥ 10) of three replicates. ^∗P < 0.05; ^∗∗P < 0.01 by Student’s t-test.