Table 1.
Sample | ka(1/Ms) | kd(1/s) | KD(M) |
---|---|---|---|
Protein SIC | 3.347 × 104 | 5.908 × 10−4 | 1.765 × 10−8 |
Histone H4 was immobilized to a Biacore CM5 Sensor Chip and different concentrations of SIC were applied in a flow. Affinity constants for SIC/histone H4 binding were determined using BIAevaluation software (GE Healthcare, Uppsala, Sweden).