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. 2018 Feb 22;9:236. doi: 10.3389/fimmu.2018.00236

Table 1.

Affinity constants for the binding of SIC to histone H4.

Sample ka(1/Ms) kd(1/s) KD(M)
Protein SIC 3.347 × 104 5.908 × 10−4 1.765 × 10−8

Histone H4 was immobilized to a Biacore CM5 Sensor Chip and different concentrations of SIC were applied in a flow. Affinity constants for SIC/histone H4 binding were determined using BIAevaluation software (GE Healthcare, Uppsala, Sweden).