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. 2018 Feb 22;10:42. doi: 10.3389/fnagi.2018.00042

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Copyright © 2018 Zhan, Stamova and Sharp.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The antibody to LPS stains LPS in human brain on Western blots and using immunofluorescence, and immunofluorescence can be eliminated by immunoprecipitating the antibody with excess LPS. Left Panel: the antibody to LPS stains one large band on a Western blot (AD (−) lane) which is eliminated when the antibody was immunoprecipitated with excess E. coli J5 LPS (AD (+) lane). The band at 150 kD in both lanes shows equal protein loading in the two lanes. Right Panel: The antibody to LPS produced more immunofluorescence in AD cortex (B) compared to control cortex (A). After immunodepletion, the immunofluorescence in AD (D) and Control (C) cortex were eliminated. These are supplementary figures from Zhan et al. (2016). Reproduced with permission.