Table 3:

Characteristics of missed spiked-in variants

Tier 1	OV10	OV11	OV12
All spiked in variants	125	116	119
Missed by VaDiR	57	55	61
Not called by at least 1 caller	20	20	20
Missed in coding region	16	17	18
Missed in coding region	11	9	13
by RNA VAF>20%
Missed in coding region	8	7	11
by RNA VAF>20%
and normal DNA DP>10
Tier 2	OV10	OV11	OV12
All spiked in variants	125	116	119
Missed by VaDiR	47	48	50
Not called by at least 1 caller	20	20	20
Missed in coding region	9	11	12
Missed in coding region	6	5	9
by RNA VAF>20%
Missed in coding region	4	4	8
by RNA VAF>20%
and normal DNA DP>10