FIG 1.

Inhibition of HCVcc entry into Huh7.5 cells by anti-HAVCR1 antibody. Huh7.5 cells were treated with decreasing concentrations of the following antibodies following 2-fold dilutions from 4 μg/ml to 0.0625 μg/ml: anti-HAVCR1 MAb (1D12) (A), anti-CD81 MAb (JS-81) (B), and isotype control (C). Cells were treated with antibodies 1 h prior to infection with J6/JFH1. After 72 h, HCVcc infection was evaluated by automatic counting of stained foci and the percentage of inhibition calculated by comparing focus counts in treated and mock-treated cells. (D) Huh7.5 cells were treated with increasing concentrations of anti-CD81 and anti-HAVCR1 MAbs individually or in combination. The percentage of inhibition and dose effect plot was created using CalcuSyn 2.0 software. Testing for all assays was performed in duplicate. Error bars represent standard errors of the means. Data are representative of three independent experiments.