FIG 7.

Phage Petty causes explosive lysis of AU0783 cells. After selecting sections of equal size (in pixels), a subset of images of single lysis events (taken at 5 frames/s) were extracted from the video microscopy using Zeiss image analysis software v. 2.3 (Zeiss, NY). The stack of images obtained was used to quantify the average intensity per pixels per frame using ImageJ (90, 91). Relative time was established using time zero as the time in the frame taken before the first lytic lesion was visible. The experiment was performed with both AU0783 cells infected by Petty (A) and E. coli cells infected by lambda (B). Each series represents one independent lysis event. The results were normalized to the average initial intensity of the images before cell lysis (before time zero).