FIG 3.

Y20 mutation alters IFITM3 subcellular localization. FLP-IN T Rex cells expressing the indicated wild-type and mutant IFITM3 proteins were cultured in the presence of Tet for 24 h to induce IFITM expression. The localization of FLAG-tagged IFITM3 (A), IFITM3/Y20A (B), and IFITM3/Y20D (C) was detected by immunofluorescent staining with an anti-FLAG monoclonal antibody (red). EEA1, Rab5, or Rab9 was visualized by immunofluorescent staining with the respective antibodies (green). Cell nuclei were stained with DAPI (blue).