FIG 3.

SBL inhibits HIV infection of macrophages at the entry level. (A) Macrophages were pretreated with GNA or SBL (50 nM) for 0.5 h (without washing it off) or 24 h (washing it off) prior to infection with DNase I-treated HIV (strain Bal). Three hours postinfection, the cellular DNA of the macrophages was harvested, and strong-stop DNA was quantified by real-time PCR. The number of copies was normalized to the control (100%). (B) Macrophages were pretreated with the indicated concentrations of SBL for 24 h prior to infection with DNase I-treated HIV (Bal). Three hours postinfection, the cellular DNA of the macrophages was harvested, and strong-stop DNA was quantified by qPCR. The data are expressed as the means and SD of the results of three independent experiments (**, P < 0.01).