FIG 17.

Trehalose, but not SMER28, induces a diffuse localization of Rab7 in infected HFFs. HFFs were mock infected (Mock) or infected with TB40E at an MOI of 0.5 (Virus) in the presence of 50 μM SMER28 or 100 mM trehalose or were left untreated and plated onto coverslips at the time of infection. At 96 hpi, coverslips were fixed and processed for immunofluorescence analysis using antibodies against IE1 72 and Rab7. Nuclei were counterstained with Hoechst 33342 dye. Images of representative cells are shown. Bars, 50 μm.