FIG 3.

Trehalose induces cytoplasmic vacuolization and alters MVB contents and intra-MVB virion morphology in HAECs. One day after replating, HAECs were mock infected (A and B) or infected with TB40E at an MOI of 3 (C to L) in the presence or absence of 100 mM trehalose. At 96 hpi, cells were fixed and processed for electron microscopy. Boxes in the left images indicate regions of enlargement in the right images. A, B, and C capsids in panels H and L are indicated by white arrowheads, black arrows, and white arrows, respectively.