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. 2018 Feb 26;92(6):e02122-17. doi: 10.1128/JVI.02122-17

FIG 2.

FIG 2

Tropism of R-313, R-315, R-317, R-319, and R-321 recombinants and, for comparison, of R-LM113 in the indicated cell lines. (A to F) The indicated cells were infected with R-313 (A), R-315 (B), R-317 (C), R-319 (D), R-321 (F), and for comparison, R-LM113 (E) at an MOI of 3 PFU/cell and monitored for EGFP expression by fluorescence microscopy 24 h postinfection. J cells express no receptor for wt HSV; J-HER2, J-nectin1, and J-HVEM express the indicated receptor. Infection was carried out in the absence of antibodies (no Ab) or in the presence of the humanized anti-HER2 monoclonal antibody trastuzumab at a concentration of 28 μg/ml. The respective adjustments for level, brightness, and contrast of each subpanel were done as follows: for R-313, a, b, e, +35 +50 +100; c, g, +35 0 +100; d, h, i, j +35 0 0; f, +0/+95 +95; k +35 +75 +100; for R-315, a, b, e, f, +35 +50 +100; c, +35 0 +100; d, h, i, j, k, +35 0 0; g, +35 0 +100; for R-317, a, b, e, f, +35 +50 +100; c, +35 0 +100; d, h, i, j, k, +35 0 0; g, +35 0 +100; for R-319, a, b, e, f, +35 +50 +100; c, +35 0 +100; d, h, i, j, k, +35 0 0; g, +35 0 +100; for R-LM113, a, b, +75 +50 +100; c, +35 0 +180; d, i, j, k, +35 0 0; e, f, +35 +50 +100; g, +35 0 +100; h, +35 −150 0; for R-321, a, b, e, f, +35 +50 +100; c, +35 0 +100; d, h, i, j, k, +35 0 0; g, +35 0 +100.