. 2018 Feb 26;92(6):e02122-17. doi: 10.1128/JVI.02122-17

TABLE 2.

Oligonucleotides employed to engineer the indicated recombinant genomes

Recombinant	GalK recombination		GCN4 recombination
Recombinant	Primer	Sequence	Primer	Sequence
R-313	gB43GalKfor	GGTGGCGTCGGCGGCTCCGAGTTCCCCCGGCACGCCTGGGGTCGCGGCCGCGCCTGTTGACAATTAATCATCGGCA	GCN4gB_43_44_fB	GGTGGCGTCGGCGGCTCCGAGTTCCCCCGGCACGCCTGGGGTCGCGGCCGCGGGATCCAAGAACTACCACCTGGAGAACGAGGTGGCCAGACTGAAGAAGCTGGTGGGCAGC
R-313	gB43GalKrev	GGCCAGGGGCGGGCGGCGCCGGAGTGGCAGGTCCCCCGTTCGCCGCCTGGGTTCAGCACTGTCCTGCTCCTT	GCN4gB_43_44_rB	GGCCAGGGGCGGGCGGCGCCGGAGTGGCAGGTCCCCCGTTCGCCGCCTGGGTGCTGCCCACCAGCTTCTTCAGTCTGGCCACCTCGTTCTCCAGGTGGTAGTTCTTGGATCC
R-315	gB81fGALK	CGGGGGACACGAAACCGAAGAAGAACAAAAAACCGAAAAACCCACCGCCGCCGCCTGTTGACAATTAATCATCGGCA	gB_81_GCN4_for	CGGGGGACACGAAACCGAAGAAGAACAAAAAACCGAAAAACCCACCGCCGCCGGGATCCAAGAACTACCACCTGGAGAACGAGGTGGCCAGACTGAAGAAGCTGGTGGGCAGC
R-315	gB81GALKrev	CGCAGGGTGGCGTGGCCCGCGGCGACGGTCGCGTTGTCGCCGGCGGGGCGTCAGCACTGTCCTGCTCCTT	gB_81_GCN4_rev	CGCAGGGTGGCGTGGCCCGCGGCGACGGTCGCGTTGTCGCCGGCGGGGCGGCTGCCCACCAGCTTCTTCAGTCTGGCCACCTCGTTCTCCAGGTGGTAGTTCTTGGATCC
R-317	gB_76_galK_for	GGCCCCGCCCCAACGGGGGACACGAAACCGAAGAAGAACAAAAAACCGAAACCTGTTGACAATTAATCATCGGCA	gB_76_GCN4_for	GGCCCCGCCCCAACGGGGGACACGAAACCGAAGAAGAACAAAAAACCGAAAGGATCCAAGAACTACCACCTGGAGAACGAGGTGGCCAGACTGAAGAAGCTGGTGGGCAGC
R-317	gB_76_galK_rev	CCCGCGGCGACGGTCGCGTTGTCGCCGGCGGGGCGCGGCGGCGGTGGGTTTCAGCACTGTCCTGCTCCTT	gB_76_GCN4_rev	CCCGCGGCGACGGTCGCGTTGTCGCCGGCGGGGCGCGGCGGCGGTGGGTTGCTGCCCACCAGCTTCTTCAGTCTGGCCACCTCGTTCTCCAGGTGGTAGTTCTTGGATCC
R-319	gB_95_galK_for	CGCCGCCGCGCCCCGCCGGCGACAACGCGACCGTCGCCGCGGGCCACGCCCCTGTTGACAATTAATCATCGGCA	gB_95_GCN4_for	CGCCGCCGCGCCCCGCCGGCGACAACGCGACCGTCGCCGCGGGCCACGCCGGATCCAAGAACTACCACCTGGAGAACGAGGTGGCCAGACTGAAGAAGCTGGTGGGCAGC
R-319	gB_95_galK_rev	GTTTGCATCGGTGTTCTCCGCCTTGATGTCCCGCAGGTGCTCGCGCAGGGTTCAGCACTGTCCTGCTCCTT	gB_95_GCN4_rev	GTTTGCATCGGTGTTCTCCGCCTTGATGTCCCGCAGGTGCTCGCGCAGGGTGCTGCCCACCAGCTTCTTCAGTCTGGCCACCTCGTTCTCCAGGTGGTAGTTCTTGGATCC