FIG 3.

(A and B) HFFs were infected with HCMV (MOI of 1) and fixed for immuno-EM at 72 hpi. The cells were further processed by primary staining with anti-MyoVa or anti-IE 1/2 (negative control) antibodies, followed by secondary staining with 10-nm protein A-gold. Imaging was conducted using a transmission electron microscope. In the rightmost image in panel A, the white arrowheads indicate capsids (without DNA) associated with MyoVa and the black arrowhead indicates a capsid (without DNA) that is not associated with MyoVa. In the rightmost image in panel B, the black arrowheads indicate capsids that are not associated with IE 1/2 (leftmost capsids contain DNA; rightmost capsid does not contain DNA). Scale bars are 100 nm. (C) The percentage of capsids associated with at least one gold particle was calculated for each condition (MyoVa, n = 95; IE 1/2, n = 127). The P value was calculated using Fisher's exact test. ****, P < 0.0001.