FIG 7.
Potency of latency reversal is correlated with survival of affected cells bearing reactivated HIV. (a) Schematic of in vitro assay to test the survival of latently infected HSPCs following treatment with LRAs. (b) Representative flow-cytometric analysis of HSPCs 1 and 4 days after treatment with LRAs. (c) Summary graph showing the relative frequency of residual PLAPpos cells in culture 4 days post-LRA treatment. (d) Summary graph showing inverse correlation between frequency of residual infected cells on 4 days after LRA treatment and potency of induced viral outgrowth by the indicated LRAs. Data were fit to a least-squares nonlinear regression. (e) Box and whisker plot (5th to 95th percentiles) of virus released into culture supernatant by HSPCs treated with TNF-α 4 days after initial LRA treatment. To facilitate comparisons across multiple experiments, data were normalized to TNF-α (n = 5). P values were calculated by two-tailed, unpaired Student's t test. *, P < 0.05; ***, P < 0.001.