FIG 2.

Viral growth kinetics of DB1-QUAD in immortalized and primary cell cultures. (A and B) Vero (A) and BEAS-2B (B) cells were infected with mKate2-labeled A2-line19F, DB1, and DB1-QUAD at an MOI of 0.01. Cells were collected at the indicated time points and titrated by FFU assay. The data represent the means ± SEM of two or three experimental replicates titrated in duplicate. (C) NHBE cells from a single donor were infected at an MOI of 2 and titrated by FFU assay. The data represent the means ± SEM of two experimental replicates titrated in duplicate. *, P < 0.05; **, P < 0.005; ****, P < 0.00005; N.S., not significant by two-way ANOVA and Tukey's multiple-comparison test. LOD, limit of detection.