Figure 2.

ATF5 is SUMOylated at a conserved SUMOylation consensus site, Lys¹⁰⁶/Lys¹⁰⁷. A, schematic of ATF5, its truncation mutants, and its SUMOylation consensus site. TAD, transcription activation domain; DBD, DNA binding domain; bZIP, basic zipper region. B, immunoblotting (IB) with an HA antibody of GFP immunoprecipitates (IP) from HeLa cells transfected with DNA empty vector (−) or expressing HA-SUMO3 (+) and other indicated DNA constructs. C and D, immunoblotting and immunoprecipitation were performed as in B, except the indicated DNA constructs were used. Pre-immune IgG (IgG) was used as a control. The arrowheads in B–D indicate the position of the expected mono-SUMOylated GFP-ATF5 at 81 kDa.