Figure 4.

SUMOylation of ATF5 interrupts ATF5 interaction with the centrosomal proteins PCNT and GCP2. A, immunoblotting (IB) and immunoprecipitation (IP) were performed using the indicated antibodies. Cell extracts were prepared from HeLa cells synchronized by DTB and release for 9, 12, and 20 h to obtain cells at G₂/M (M), Anaphase (Ana), and G₁ phase cells. Cyclin E and cyclin B were used as G₁ and M phase markers, respectively. B–D, immunoblotting and immunoprecipitation were performed as in A, except the indicated antibodies were used. Cell extracts were prepared from HeLa cells transfected with the indicated DNA constructs.