Figure 1.
Folded state and orientation of the Bam complex following reconstitution into proteoliposomes that contain distinct phospholipids. A, chemical structures of the synthetic lipids used in this study and their phase transition temperatures (Tm). B, proteoliposomes that were generated using the purified Bam complex and the indicated lipids were untreated, heated in SDS sample buffer, or treated with trypsin and subjected to SDS-PAGE at 4 °C. Proteins were visualized by Coomassie Blue staining. A trypsin-protected species that probably corresponds to the β barrel domain of BamA (BamA*) is highlighted. The small BamE protein was difficult to visualize on some of the gels and is not shown here.