Figure 7.

Jabba and CK2 regulate lipid metabolism during Drosophila oogenesis. A, ovaries from flies of the indicated genotypes were dissected and stained with Oil Red O for visualization of neutral lipids. Shown are representative stage 10 egg chambers. Scale bar, 100 μm. B, quantitation of total triglyceride levels from ovaries of flies of the indicated genotypes. Data are presented as the mean (n = 4) with error bars denoting S.D. * indicates a p value <0.05. ns, not significant. C, confocal immunofluorescence microscopy of egg chambers from flies of the indicated genotypes. Dissected egg chambers were fixed and stained with anti-myc antibody to detect Jabba-labeled lipid droplets (green) and counterstained with rhodamine phalloidin to label F-actin (red). Shown are single optical confocal sections. Scale bars, 50 μm.