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Closed and open conformation–specific sABs are competitive. A, single-point ELISA shows that the binding of sAB-7O and sAB-11M are exclusive. Neither of them binds in the presence of an excess of the other. B, competitive ELISA showing sAB-7O displacing His6-sAB-11M from MBP saturated with His6-sAB-11M. In both A and B, the sAB of interest was His-tagged, and the ELISA signal (A450) was monitored using anti-His antibody. Error bars, S.D.
