Table 2.
Thermodynamic parameters from ITC experiments at 25 °C
| Titration | ΔH0 | −TΔS0 | ΔG0 | KD = 1/KB |
|---|---|---|---|---|
| kcal mol−1 | kcal mol−1 | kcal mol−1 | nm | |
| Maltose: MBP | 2.1 ± 0.1 | −10.4 ± 0.1 | −8.3 ± 0.1 | 830.0 |
| MBP: sAB-11M in 1 mm maltose | −14 ± 2 | 2 ± 2 | −11.4 ± 0.1 | 4.0 |
| Maltose: MBP in 5-fold molar excess of sAB-11M | −8.9 ± 0.8 | −2 ± 1 | −11.1 ± 0.2 | 7.1 |
| MBP: sAB11M(calculated) | −3 ± 2 | −5.6 ± 2 | −8.6 ± 0.2 | 470.0 |
| MBP: sAB-P1 in 1 mm maltose | −16.8 ± 0.5 | 5.8 ± 0.7 | −11.0 ± 0.2 | 9.0 |
| Maltose: MBP in 5-fold molar excess of sAB-P1 | −14 ± 2 | 1 ± 2 | −12.6 ± 0.1 | 0.6 |