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Kinetic parameters of binding of WT sAB-11M and Ala mutants with WT MBP
| Variants of sAB-11M | KD in 1 μm maltose | KD in 1 mm maltose | ΔΔG0 |
|---|---|---|---|
| nm | nm | kcal mol−1 | |
| WT | 20.0 | 1.5 | −1.5 |
| Y57A | 104.5 | 7.6 | −1.5 |
| R67A | 305.0 | 20.0 | −1.6 |
| W112A | 350.0 | 29.4 | −1.5 |
| P113A | 715.0 | 63.0 | −1.4 |
