Figure 4.

Kinetic analysis of TSTD1 thiosulfate:cyanide sulfurtransferase activity. The dependence of the reaction velocity on cyanide concentration (0.02–50 mm) in the presence of 50 mm thiosulfate (A) and on thiosulfate concentrations (1–50 mm) in the presence of 30 mm cyanide (B) were determined. The data were fitted with the Michaelis–Menten equation. Data represent the mean ± S.D. (error bars) of three independent experiments performed in 300 mm HEPES, pH 7.4, 150 mm NaCl.