Figure 6.

Kinetic analysis of TSTD1 thiosulfate-thioredoxin sulfur transfer activity. TSTD1 sulfur transfer activity to thioredoxin was determined in the presence of 50 mm thiosulfate and varying concentrations of thioredoxin (A) and 150 μm thioredoxin and varying concentrations of thiosulfate (B). Reactions were performed as described under “Experimental Procedures” and fitted with the Michaelis–Menten equation. Data represent the mean ± S.D. (error bars) of three independent experiments performed in 300 mm HEPES, pH 7.4, 150 mm NaCl.