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Assay for TLS opposite (r) γ-HOPdG. A, structure of reduced γ-HOPdG. B, the target 16-mer sequence containing (r) γ-HOPdG nt at G* is shown on top, and the lacz′ sequence in the leading strand in the pBS vector containing the adduct at G* is shown below. The (r) γ-HOPdG–containing DNA strand is in frame, and it carries the Kan+ gene. TLS through the adduct generates Kan+ blue colonies.
