Table 4.
Effect of siRNA knockdown of Rev1 alone or together with Polη on TLS opposite (r) γ-HOPdG present on the leading strand template in wildtype human fibroblasts carrying a vector expressing the siRNA-resistant (siR) form of wildtype or catalytically inactive Rev1
| siRNA | Vector-expressing | No. of Kan+ colonies | No. of blue colonies among Kan+ | TLS |
|---|---|---|---|---|
| % | ||||
| Rev1 | Vector control | 325 | 39 | 12.0 |
| Rev1 | FLAG-WT-Rev1 | 384 | 45 | 11.7 |
| Rev1 | FLAG-WT-siR-Rev1 | 326 | 104 | 31.9 |
| Rev1 | FLAG-D570A/E571A-siR-Rev1 | 358 | 79 | 22.1 |
| Polη + Rev1 | FLAG-WT-Rev1 | 348 | 40 | 11.5 |
| Polη + Rev1 | FLAG-WT-siR-Rev1 | 316 | 75 | 23.7 |
| Polη + Rev1 | FLAG-D570A/E571A-siR-Rev1 | 372 | 81 | 21.8 |