Domain architecture and hydrolytic activities of chitinases.
A, domain architecture of the enzyme used in this study. Active catalytic domains, inactive catalytic domains, chitin-binding domains, and linker regions are highlighted in orange, green, blue, and white, respectively. B, the hydrolytic activities of chitinases toward different substrates. Products were measured during degradation of polymer chitin substrates, colloidal chitin (left panel), α-chitin (middle panel), and β-chitin (right panel). The reaction mixtures contained identical amounts of enzyme and substrate. The results are the average of three independent repeats, with the standard deviations indicated.