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. 2018 Feb 26;8:3607. doi: 10.1038/s41598-018-22064-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cerebral cortex stained for glucose transporter 1 (Glut1) of surviving mice. (a) Representative images of Glut1-immunostained sections of cerebral cortex of WT, KO and EC-Tg mice at 28 days after BCAS. Scale bar represents 100 μm. (b) Quantified results of cerebral microvessels. A column with a bar represents mean ± SD of the number of cerebral microvessels/mm². There was a significant difference in the number of cerebral microvessels between the three genotypes using one-way ANOVA (WT, n = 6; KO, n = 5; EC-Tg, n = 8; p = 0.002). There were significant differences between KO and WT (p = 0.034) and between EC-Tg and WT (p = 0.001) using Tukey’s test; *p < 0.05 vs WT.