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. 2018 Feb 26;8:3644. doi: 10.1038/s41598-018-21990-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inter-group comparison of FREE2 DNA methylation in buccal epithelial cells. Controls: control participants with normal FMR1 CGG size (<45 CGG); PM/FM: participants with FMR1 PM/FM (premutation/full mutation) CGG size mosaicism; FM: participants with FMR1 FM CGG expansion only. Note: the parallel black broken lines at 0.1 MOR represents the analytical sensitivity of the EpiTYPER assay, as previously defined⁴; ****p < 1.3 × 10⁻⁶ for the comparison between controls and participants with FM expansion (see Supplementary Table S5); ***p < 0.0005 for the comparison between controls and participants with PM/FM size mosaicism (see Supplementary Table S6); **p < 0.001 for the comparison between controls and participants with PM/FM size mosaicism (see Supplementary Table S6); ^##p < 0.001 for the comparison between participants with PM/FM size mosaicism and participants with FM only (see Supplementary Table S3); ^#p < 0.005 for the comparison between participants with PM/FM size mosaicism and participants with FM only (see Supplementary Table S3).