Table 2.
Comparison of FREE2 DNA methylation in buccal epithelial cells between the FXS and control group.
| FXS | Controls | FXS vs Controls | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| n | Median (IQR) | Mean (SD)+ | Min-Max | n | Median (IQR) | Mean (SD) | Min-Max | p-value* | p-value** | |
| MOR CpG1 | 25 | 0.860 (0.170) | 0.825 (0.134) | 0.020–0.975 | 16 | 0.027 (0.038) | 0.039 (0.038) | 0.005–0.130 | 3.2 × 10−7 | 1.2 × 10−23 |
| MOR CpG2 | 25 | 0.715 (0.150) | 0.730 (0.144) | 0.015–0.965 | 15 | 0.000 (0.010) | 0.009 (0.015) | 0.000–0.055 | 2.9 × 10−7 | 8.6 × 10−21 |
| MOR CpG6/7 | 24 | 0.804 (0.139) | 0.787 (0.097) | 0.060–0.900 | 18 | 0.064 (0.045) | 0.070 (0.028) | 0.025–0.130 | 2.9 × 10−7 | 5.0 × 10−28 |
| MOR CpG8/9 | 25 | 0.750 (0.135) | 0.716 (0.129) | 0.080–0.950 | 19 | 0.050 (0.020) | 0.052 (0.023) | 0.010–0.105 | 1.3 × 10−7 | 4.5 × 10−24 |
| MOR CpG10-12 | 25 | 0.760 (0.115) | 0.735 (0.126) | 0.040–0.890 | 16 | 0.010 (0.006) | 0.015 (0.010) | 0.005–0.045 | 2.2 × 10−7 | 1.2 × 10−23 |
Abbreviations: n, number of participants’ samples from which MALDI-TOF MS results were available; IQR, interquartile range; SD, standard deviation; Min – Max = minimum and maximum values; MOR, methylation output ratio; Adjusted p-values (for multiple testing using FDR) computed using *Mann-Whitney test to compare the median between the two groups and **two-sample t-test to compare the mean between the two groups, where + mean and SD in the FXS group are computed with one outlier excluded; p-values less than 0.05 are highlighted in bold.