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. 2018 Feb 27;6(5):e13583. doi: 10.14814/phy2.13583

Figure 3.

Figure 3

Skeletal muscle Akt phosphorylation in response to insulin in CFO and HFO. Soleus muscle Akt phosphorylation on sites threonine 308 (A) and serine 473 (B) in response to basal and insulin stimulation conditions in CFO (black bars) and HFO (white bars). Soleus AS160 protein representative blots for CFO and HFO at 6 weeks, 6 months, and 12 months (C). Six weeks (CFO: n = 8, three females, five males; HFO: n = 6, two females, four males), 6 months (CFO: n = 6, three females, three males; HFO: n = 6, three females, three males), 12 months (CFO: n = 6, three females, three males; HFO: n = 6, three females, three males). Offspring were randomized across age groups from CFO (six litters) and HFO (four litters). *Significant difference between HFO and CFOs at similar age and insulin condition (P < 0.05). aSignificant difference between 6 months and 6 weeks for that paternal group (P < 0.005). bSignificant difference between 12 months and 6 weeks for that paternal group (P < 0.01). cSignificant difference between 12 months and 6 months for that paternal group (P < 0.05).