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. 2018 Feb 19;9:268. doi: 10.3389/fimmu.2018.00268

Figure 1.

Figure 1

Glutathione S-transferase Pi (GSTP) improves survival and reduces serum high mobility group box-1 protein (HMGB1) level in septic mice. (A) Gstp−/− and wild type C57BL/6 mice were treated with 2 mg/kg of GSTP or lypopolysaccharide-free saline, respectively, 30 min after CLP surgery, and survival rate of mice was monitored continuously. (B) Lung tissues of the mice were collected and subjected to Hematoxylin and Eosin staining 10 h after CLP, and examined by light microscopy (magnification, ×100). Scale bar: 100 µm. Histological scoring of lung injury was evaluated based on inflammatory infiltrate in CLP mice. (C) Detection of mouse serum HMGB1 level by ELISA. Mouse blood was collected from retroorbital sinuses of mice. Serum HMGB1 level was measured by ELISA 12 h after CLP surgery. Data information: in (A), Kaplan–Meier analysis was used to analyze the survival rates of CLP mice. n = 10 mice/group. **P<0.01 versus Gstp−/− CLP group by the log-rank test for between-group comparisons. In (B,C), Graphs show mean ± SD. n = 8–10 mice/group. **P<0.01 versus corresponding CLP group by unpaired Student’s t-test. Data shown were representative of three independent experiments.