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. 2018 Feb 19;9:280. doi: 10.3389/fimmu.2018.00280

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Copyright © 2018 Arrieta-Bolaños, Crivello, Metzing, Meurer, Ahci, Rytlewski, Vignali, Yusko, van Balen, Horn, Falkenburg and Fleischhauer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expansion of DPB1-specific alloreactive clones detected in pre-culture samples. Fold expansion among alloreactive T cell clones present in the cultured samples and that could be detected in the pre-culture samples for each responder (R) was quantified and plotted (y axis) against their frequency in the pre-culture sample (x axis). The size of the circle represents the share of each clone in the total cultured set. Highest values (%) are indicated for each sample. Left panels, clones responding against DPB1*02:01; right panels, clones responding against DPB1*09:01. As shown in Figure 7A (purple dots), 177, 146, and 92 clones against DPB1*02:01, and 249, 43, and 112 clones against DPB1*09:01 were detected in both the cultured and the pre-culture samples for R1, R2, and R3, respectively.