Table 2.

Computational methods for circulating tumor cell (CTC) analysis based on single-cell-sequencing approaches.

Computational method	Technical challenges	Target	Reference
Single-cell DNA sequencing	–Low coverage –Nonuniform coverage –PCR errors –Allele dropout –Allelic imbalance	SNV	(39)
		CNV	(42–46)
		Phylogeny	(51, 52, 54–56)
Single-cell RNA sequencing	–Amplification bias –Dropout of low abundant transcripts	Gene expression profiling	(60–62, 67, 69)
		Differential expression	(63, 64, 68)
		Coexpression network	(76)
		Phylogeny	(47, 48, 69–75)
		SNV	(49)
		CNV	(47, 48)

Computational methods for CTC analysis, related technical challenges and applications (target), as well as references are shown.

SNV, single nucleotide variant; CNV, copy number variant.