Figure 2.

RT-PCR screening for endocrine perturbation in NGN3-edited E60 fetuses. (A) Schematic showing exocrine and endocrine lineage specification in the pancreas, and representative marker genes expressed in respective precursor or specialized cell types. (B) Combined 2% agarose gel images from amplicons using RT-PCR from pancreas tissue cDNA. Gene-specific primers were used following first strand-synthesis (OligodT) for NEUROD1, PAX4, NKX2.2, INS, SST, GCG, PPY, AMY, CK19. Fetuses #2, #3, #4, #5, #7 and WT control were used for representation of pancreas gene profiles following NGN3-gene targeting. Cropped and merged image boundaries are depicted by the white borders.