Figure 5.

APOL1-G0, -G1, -G2 cause a time-dependent loss of cellular K⁺ and a gain of cellular Na⁺. Cell-associated (A) K⁺ and (C) Na⁺ were measured as described in the Concise Methods in G0, G1, or G2 cells without or with tetracycline (1 µg/ml) for 4 hours (mean±SD, n=5–6). Cellular (B) K⁺ and (D) Na⁺ content as a function of time after tetracycline addition is shown, normalized to time 0 (mean±SD, n=3). (B) Hatches between the time 0 and the 5-hour time measurement added for clarity, illustrating this line connects these two time points and does not represent additional interval measurements. Tet, tetracycline.