Figure 2.

Characterization of THP in the circulation. Western blots for THP after immuno-precipitation are shown in all lanes except lane c. Lanes a and b demonstrate that THP in the human (h) serum has a comparable molecular mass (approximately 85–90 kD) to full-length human urinary THP (lane c). Lanes d–g show immuno-blotting for THP on different fractions of serum, separated according to molecular mass by size exclusion chromatography. THP is predominantly present in the fractions between 44 and 158 kD (lane f, asterisk), proving that THP in the circulation is mostly in the monomeric form.