Figure 2.

Confirmation of DCT or CCD enriched proteins in vivo. (A) Confocal images of mouse kidney sections immunolabeled for COP9 Signalosome Subunit 6 (CSN6), USP32, FBXO2, and CHIP alongside the DCT- or CCD-expressed proteins NCC or AQP2, respectively. (B) Immunoblotting of purified DCT cells (green fluorescent protein positive [GFP⁺]) illustrates that mpkDCT-enriched proteins FBXO2, SMAD4, SIX4, and FBXO29 are abundant in isolated DCT cells, whereas the mpkCCD-enriched proteins NRK1, USP4, CSN6, and CHIP are predominantly identified in the GFP⁻ fraction (representing non-DCT cells).