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CHIP can ubiquitiylate AQP2 in vitro. Immunoblot analysis of in vitro ubiquitylation reactions in the presence or absence of the various recombinant proteins indicated, including ubiquitin (Ub). The target of antibody used in the immunoblot (IB), molecular mass markers (kilodaltons), and the presence of oligomeric AQP2 (AQP2n) and polyubiquitylated AQP2 (AQP2Ub) are specified.
