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. 2018 Feb 15;29(3):806–824. doi: 10.1681/ASN.2017080890

Figure 3.

Figure 3.

Transcriptional profiling of mouse and putative human NPCs assisted by intracellular staining of Six2/SIX2 followed by FACS (MARIS). (A) Separation of Six2+ cell population from dissociated mouse (m) embryonic kidney cortex cells by either FACS of Six2GFP reporter line (middle) or Six2 MARIS (right). (B) Gene-level correlation of normalized mRNA-Seq reads between NPC profiles generated by Six2 reporter line (Six2GFP+) and Six2 MARIS (mSIX2+). (C) Overlap (left) between NPC-specific genes identified by differential gene expression analysis between Six2GFP+ versus Six2GFP− (TPM_Six2GFP+>5, TPM_Six2GFP+/TPM_Six2GFP−>3, P<0.05), or between mSix2+ versus mSix2− (TPM_mSix2+>5, TPM_mSix2+/TPM_Six2−>3, P<0.05). Results (middle) of GO term enrichment analysis of the indicated gene sets, with representative ones (right) from each set of genes. (D) Separation of SIX2+ cell population from dissociated human (hu) fetal kidney cortex. (E) Gene-level correlation of normalized mRNA-seq reads between human and mouse NPC profiles obtained by MARIS; human (orange) or mouse (cyan) enriched genes were indicated. (F) Top three GO terms enriched from the human (top) and mouse (bottom) enriched genes. Ab, antibody; enrich., enriched; norm., normalized vs., versus.