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Validation of NPC cell populations and exploration of novel NPC marker genes. (A) Genes identified in NPC subclusters stratify into distinct gene expression patterns; section in situ hybridization for genes as specified on fields, clusters as specified. (B) Gene expression plot for TMEM100, ROBO2, and CITED1. (C) Immunofluorescent staining for TMEM100, CITED1, ROBO2, and KRT8/19 in human fetal kidney. Scale bar as indicated. RV, renal vescile UB, ureteric bud epithelium
