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RNase E depletion causes abnormal morphology of EHEC cells. (A) TEA028; (B, C, D) TEA028-rne. Bacteria were grown overnight in LB medium either in the absence of IPTG (A) or in the presence of 0.1 μM (B), 100 μM (C) or 500 μM (D) IPTG. Smears were stained with crystal violet. At the lowest IPTG concentration (0.1 μM), TEA028-rne cells grew abnormally as filaments. Magnification = ×1000. Bar: 5 μM.
