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. 2018 Feb 26;8:3624. doi: 10.1038/s41598-018-21812-0

Figure 2.

Figure 2

Smad2/3 mediates TGF-β1-induced alterations of miR-206, HDAC4 and cyclin D1. ASMCs were treated with SB431542 (10 μM) for 1 h before stimulation with TGF-β1 (10 ng/ml) for 24 h. (a) The level of miR-206 was measured using qRT-PCR. U6 small nuclear RNA served as a loading control (n = 4 per group). (b) HDAC4 protein level was analyzed using immunoblotting (n = 4 per group). (c) Cyclin D1 protein level was determined using immunoblotting (n = 4 per group). The full-length blots of Fig. 2b and 2c are presented in Supplementary Fig. S1. *P < 0.01 versus control. #P < 0.01 versus TGF-β1-treated cells.