Skip to main content
. 2018 Feb 26;8:3624. doi: 10.1038/s41598-018-21812-0

Figure 6.

Figure 6

The mechanisms underlying activation of AMPK inhibition of TGF-β1-induced ASMCs proliferation. (a) ASMCs were transfected with AMPK α2-specific or non-targeting siRNA for 48 h, and then treated with metformin (10 mM) for 6 h before stimulation with TGF-β1 (10 ng/ml) for 1 h. The phosphorylation of Smad2/3 was determined by immunoblotting (n = 4 per group). ASMCs were transfected with AMPK α2-specific or non-targeting siRNA for 24 h, and then treated with metformin (10 mM) for 6 h before stimulation with TGF-β1 (10 ng/ml) for 24 h. (b) The expression of miR-206 was examined by qRT-PCR (n = 4 per group). (c) The expression and phosphorylation of HDAC4 were analyzed using immunoblotting (n = 4 per group). (d) Cyclin D1 protein level was determined using immunoblotting (n = 4 per group). The full-length blots of Fig. 6a, 6c and 6d are presented in Supplementary Fig. S4. *P < 0.05 versus control. **P < 0.01 versus control. #P < 0.01 versus TGF-β1-treated cells. &P < 0.01 versus metformin and TGF-β1-stimulated cells.