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. 2018 Feb 26;9:825. doi: 10.1038/s41467-018-03062-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The Stormorken R304W STIM1 mutant is constitutively active. All experiments performed with HEK 293 cells. a Patch clamp recordings of CFP-STIM1 + YFP-Orai1 (black) and Stormorken mutant CFP-STIM1 R304W + YFP-Orai1 (red). b Representative images of co-localization experiments using confocal fluorescence imaging of CFP-Orai1 + YFP-STIM1 (left) and CFP-Orai1 + YFP-STIM1 R304W (right), both in resting (−TG) as well as store depleted (+TG) state. Length of scale bars correspond to 5 µm. c Representative images of localization experiments using confocal fluorescence imaging of YFP-STIM1 (top) and YFP-STIM1 R304W (bottom), both in resting (−TG) as well as store depleted (+TG) state. Length of scale bars correspond to 5 µm. d FRET homomerization experiments of YFP- + CFP- labeled STIM1 (black) and YFP- + CFP-labeled STIM1 R304W (red) in response to stored depletion using 2 µM TG. e Expression levels of STIM1 wt and STIM1 R304W, respectively, in HEK 293 cells. f FRET homomerization experiments of YFP-OASF + CFP-OASF (black) and YFP-OASF R304W + CFP-OASF R304W (red), respectively. *Significant difference (p < 0.05). Error bars are defined as SEM. Statistics are Student’s t‐test