Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 26;9:825. doi: 10.1038/s41467-018-03062-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — High specificity of STIM1 amino acid position 304. All experiments performed with HEK 293 cells. a Bar diagram of FRET measurements of conformational sensor YFP-OASF-CFP wt (black) and mutants (L300W (magenta), L303W (green), R304W (red), E305W (blue), E308W (violet)). *Significant difference (p < 0.05) to wt. n-number in brackets. b Patch clamp recordings of YFP-Orai1 + CFP-STIM1 wt (black) and mutants (color code corresponds to a). c Representative confocal images of cells co-expressing CFP-STIM1 R303W + YFP-Orai1 (left) and CFP-STIM1 R304W + YFP-Orai1 (right) in resting (−TG) as well as store-depleted (+TG) conditions. Merge shows localization of both CFP-STIM1 and YFP-Orai1 signals in one cell. Length of scale bars correspond to 5 µm. d Bar diagram representing co-localization experiments using confocal microscopy of HEK293 cells co-expressing YFP-Orai1 and CFP-STIM1 wt and mutants, respectively, in both resting (−TG) as well as store-depleted (+TG) conditions (color code corresponds to a). *Significant difference (p < 0.05) to wt (only – TG). Error bars are defined as SEM. Statistics are Student’s t‐test