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. 2018 Feb 26;8:3635. doi: 10.1038/s41598-018-21650-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Calcified Wall Thickness of Abdominal Aorta of MGP Genotypes at 25 days of Age. MGP heterozygous males and females were mated. At the start of pregnancy dams were fed either a control or a high-fat diet. These diets were continued throughout pregnancy and after birth. Wild type (WT) and MGP knockout (KO) offspring were analysed at 25 days of age. The thickness of calcification of the abdominal aorta wall was determined by CT. The section of the aorta from the diaphragm to the split for the femoral arteries was analysed. Bars show calcified wall thickness for each MGP genotype and diet. Graphs show by sex, n = 4–6 per group. Graphs show mean plus 95% confidence limits.