Table 3.
Mean Normalised Gene Expression Levels in Femur.
| Sex | Males | Females | ||||||
|---|---|---|---|---|---|---|---|---|
| Genotype | WT | KO | WT | KO | ||||
| Mean (SD) | Mean (SD) | Mean (SD) | Mean (SD) | |||||
| Diet | Control | High Fat | Control | High Fat | Control | High Fat | Control | High Fat |
| (n = 8) | (n = 14) | (n = 8) | (n = 7) | (n = 11) | (n = 11) | (n = 9) | (n = 6) | |
| Osteocalcin | 0.20 (0.11) | 0.25 (0.07) | 0.25 (0.19) | 0.08 (0.01) | 0.19 (0.08) | 0.19 (0.09) | 0.14 (0.11) | 0.08 (0.02) |
| Gas6 | 0.03 (0.02) | 0.04 (0.03) | 0.06 (0.02) | 0.04 (0.01) | 0.04 (0.02) | 0.04 (0.01) | 0.06 (0.03) | 0.03 (0.02) |
| Periostin | 2.4 (1.1)ab | 2.4 (1.1)b | 0.7 (0.4)c | 1.1 (0.4)ac | 2.9 (0.9)a | 2.4 (1.1)ac | 0.7 (0.3)bc | 1.5 (1.1)c |
| Mgp | 0.078 (0.044)a | 0.061 (0.030)a | 0.004 (0.003)b | 0.007 (0.003)b | 0.093 (0.053)a | 0.055 (0.025)ab | 0.004 (0.003)b | 0.003 (0.002)b |
| Ggcx | 0.0014 (0.0007) | 0.0013 (0.0005) | 0.0010 (0.0004) | 0.0010 (0.0003) | 0.0012 (0.0006)ab | 0.0013 (0.0004)a | 0.0006 (0.0004)b | 0.0010 (0.0002)ab |
| Vkor | 0.022 (0.010) | 0.026 (0.012) | 0.014 (0.007) | 0.014 (0.003) | 0.026 (0.012)a | 0.019 (0.007)ab | 0.012 (0.004)b | 0.014 (0.005)ab |
| Bmp-1 | 0.7 (0.4) | 0.8 (0.3) | 0.6 (0.3) | 0.6 (0.4) | 0.7 (0.3) | 0.6 (0.2) | 0.4 (0.1) | 0.6 (0.3) |
| Bmp-2 | 0.004 (0.001)ab | 0.005 (0.003)ab | 0.007 (0.002)a | 0.003 (0.003)b | 0.005 (0.003) | 0.004 (0.003) | 0.003 (0.002) | 0.005 (0.003) |
| Bmp-4 | 0.05 (0.02) | 0.06 (0.03) | 0.08 (0.03) | 0.06 (0.03) | 0.06 (0.04) | 0.05 (0.02) | 0.08 (0.05) | 0.03 (0.02) |
| Leptin | 0.019 (0.014) | 0.023 (0.020) | 0.020 (0.013) | 0.021 (0.009( | 0.015 (0.007) | 0.024 (0.011) | 0.023 (0.018) | 0.026 (0.026) |
| Leptin Receptor | 0.33 (0.04) | 0.36 (0.28) | 0.57 (0.23) | 0.46 (0.20) | 0.53 (0.32) | 0.33 (0.16) | 0.49 (0.28) | 0.35 (0.12) |
| Sclerostin | 0.0013 (0.0004) | 0.0025 (0.0011) | 0.0021 (0.0010) | 0.0021 (0.0008) | 0.0023 (0.0012) | 0.0019 (0.0008) | 0.0021 (0.0015) | 0.0023 (0.0013) |
| Estradiol | ND | ND | ND | ND | ND | ND | ND | ND |
| Estradiol Receptor α | 0.13 (0.05) | 0.13 (0.04) | 0.10 (0.04) | 0.12 (0.05) | 0.16 (0.07)a | 0.13 (0.05)ab | 0.06 (0.03)b | 0.09 (0.03)ab |
| Estradiol Receptor β | ND | ND | ND | ND | ND | ND | ND | ND |
| ACE | 0.31 (0.07) | 0.38 (0.24) | 0.22 (0.08) | 0.34 (0.10) | 0.32 (0.09) | 0.29 (0.12) | 0.19 (0.09) | 0.22 (0.09) |
| Angiotensinogen | 0.16 (0.04) | 0.26 (0.17) | 0.33 (0.17) | 0.31 (0.18) | 0.29 (0.18) | 0.19 (0.09) | 0.32 (0.17) | 0.21 (0.04) |
| Vegf 120 | 0.05 (0.02) | 0.07 (0.02) | 0.08 (0.03) | 0.10 (0.07) | 0.08 (0.08) | 0.08 (0.03) | 0.08 (0.05) | 0.05 (0.03) |
| Vegf 164 | 0.08 (0.03) | 0.12 (0.06) | 0.08 (0.02) | 0.11 (0.05) | 0.11 (0.04) | 0.13 (0.05) | 0.07 (0.03) | 0.08 (0.04) |
| Vegf 188 | 0.02 (0.01) | 0.02 (0.02) | 0.02 (0.01) | 0.01 (0.010) | 0.04 (0.04) | 0.02 (0.01) | 0.01 (0.01) | 0.01 (0.00) |
| Vegf total | 0.11 (0.03) | 0.15 (0.06) | 0.12 (0.04) | 0.10 (0.02) | 0.16 (0.07) | 0.15 (0.05) | 0.10 (0.04) | 0.10 (0.02) |
| Gapdh | 14 (11) | 7 (5) | 12 (8) | 12 (9) | 10 (7) | 9 (3) | 11 (5) | 9 (6) |
For each parameter measured, values with different superscript letters are significantly different from each other within the same sex (p < 0.05). ND – not detected.