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. 2018 Feb 26;8:3626. doi: 10.1038/s41598-018-21882-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Human RNase P (hRPP30) gene target template sequence (Accession number NC_018921). (A) hRPP30 79 base long amplicon spanning five bases in 5′ UTR region and 74 bases in the region (90871987 to 90872071) that encodes a part of the first exon and located at 10q23.31 on the chromosome 10. (B) Various domains in human RPP30 mRNA isoforms are shown. Translation initiation site (green) and translation termination site (red) arrows and protein encoding (blue colored) region are shown. (C) Clustal Omega multiple sequence alignment analysis of RPP30 amplicon’s genomic sequences in humans and non-human primate species. Forward primer (FP, green), reverse primer (RP, dark red) and probe (blue) conserved sequences and non-homologous mutations (orange) in human and various non-human primate species are shown.