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. 2018 Feb 22;12:43. doi: 10.3389/fncel.2018.00043

Table 1.

Primary and secondary antibodies used.

Antigen Species of origin Company/Order No. Dilution (DAB/FL) Address of company
Primary antibodies
Fos gt Santa Cruz/sc-52 1:2000/1:100 Santa Cruz Biotechnology, Inc., Dallas, TX, United States
GFAP mo Sigma/G3893 1:5000/1:5000 Sigma–Aldrich Chemie GmbH, Munich, Germany
GAD65 mo Millipore/MAB351 1:2000/1:500 Merck Chemicals GmbH, Darmstadt, Germany
GAD67 mo Millipore/MAB5406 1:5000/1:500 Merck Chemicals GmbH, Darmstadt, Germany
vGluT1 gp Millipore/AB5905 1:5000/1:1000 Merck Chemicals GmbH, Darmstadt, Germany
IBA1 rb Wako/019-19741 1:1000/1:500 Wako Pure Chemical Industries, Ltd., Osaka, Japan
HuC/HuD mo Invitrogen/A-21271 - /1:100 Thermo Fisher Scientific Inc., Darmstadt, Germany
GABA rb Sigma/A2052 - /1:2000 Sigma–Aldrich Chemie GmbH, Munich, Germany
S100 rb Dako/Z0311 - /1:400 DAKO, Agilent, Santa Clara, CA, United States
Secondary antibodies for DAB staining
gt IgG rb/HRP Vector/BA-5000 1:200 Vector Laboratories, Burlingame, CA, United States
mo IgG ho/HRP Vector/BA-2001 1:200 Vector Laboratories, Burlingame, CA, United States
rb IgG gt/HRP Vector/BA-1000 1:200 Vector Laboratories, Burlingame, CA, United States
gp IgG gp/HRP Vector/BA-5003 1:200 Vector Laboratories, Burlingame, CA, United States
Secondary antibodies for immunofluorescence staining
gt IgG dk/Cy3 Millipore/AP180C 1:200 Merck Chemicals GmbH, Darmstadt, Germany
rb IgG dk/Alexa488 Molecular Probes/A-21206 1:200 Thermo Fisher Scientific Inc., Darmstadt, Germany
mo IgG dk/Alexa488 Molecular Probes/A-21202 1:200 Thermo Fisher Scientific Inc., Darmstadt, Germany
rb IgG gt/Biotin Vector/BA-1000 1:200 Vector Laboratories, Burlingame, CA, United States
Streptavidin Amca Vector/SA-5008 1:33 Vector Laboratories, Burlingame, CA, United States