Primary antibodies |
Fos |
gt |
Santa Cruz/sc-52 |
1:2000/1:100 |
Santa Cruz Biotechnology, Inc., Dallas, TX, United States |
GFAP |
mo |
Sigma/G3893 |
1:5000/1:5000 |
Sigma–Aldrich Chemie GmbH, Munich, Germany |
GAD65 |
mo |
Millipore/MAB351 |
1:2000/1:500 |
Merck Chemicals GmbH, Darmstadt, Germany |
GAD67 |
mo |
Millipore/MAB5406 |
1:5000/1:500 |
Merck Chemicals GmbH, Darmstadt, Germany |
vGluT1 |
gp |
Millipore/AB5905 |
1:5000/1:1000 |
Merck Chemicals GmbH, Darmstadt, Germany |
IBA1 |
rb |
Wako/019-19741 |
1:1000/1:500 |
Wako Pure Chemical Industries, Ltd., Osaka, Japan |
HuC/HuD |
mo |
Invitrogen/A-21271 |
- /1:100 |
Thermo Fisher Scientific Inc., Darmstadt, Germany |
GABA |
rb |
Sigma/A2052 |
- /1:2000 |
Sigma–Aldrich Chemie GmbH, Munich, Germany |
S100 |
rb |
Dako/Z0311 |
- /1:400 |
DAKO, Agilent, Santa Clara, CA, United States |
Secondary antibodies for DAB staining |
gt IgG |
rb/HRP |
Vector/BA-5000 |
1:200 |
Vector Laboratories, Burlingame, CA, United States |
mo IgG |
ho/HRP |
Vector/BA-2001 |
1:200 |
Vector Laboratories, Burlingame, CA, United States |
rb IgG |
gt/HRP |
Vector/BA-1000 |
1:200 |
Vector Laboratories, Burlingame, CA, United States |
gp IgG |
gp/HRP |
Vector/BA-5003 |
1:200 |
Vector Laboratories, Burlingame, CA, United States |
Secondary antibodies for immunofluorescence staining |
gt IgG |
dk/Cy3 |
Millipore/AP180C |
1:200 |
Merck Chemicals GmbH, Darmstadt, Germany |
rb IgG |
dk/Alexa488 |
Molecular Probes/A-21206 |
1:200 |
Thermo Fisher Scientific Inc., Darmstadt, Germany |
mo IgG |
dk/Alexa488 |
Molecular Probes/A-21202 |
1:200 |
Thermo Fisher Scientific Inc., Darmstadt, Germany |
rb IgG |
gt/Biotin |
Vector/BA-1000 |
1:200 |
Vector Laboratories, Burlingame, CA, United States |
Streptavidin |
Amca |
Vector/SA-5008 |
1:33 |
Vector Laboratories, Burlingame, CA, United States |